4pt4

X-ray diffraction
2.04Å resolution

Crystal structure Analysis of N terminal region containing the dimerization domain and DNA binding domain of HU protein(Histone like protein-DNA binding) from Mycobacterium tuberculosis [H37Ra]

Released:

Function and Biology Details

Reaction catalysed:
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-217962 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding protein HupB Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 10.67 KDa
Source organism: Mycobacterium tuberculosis H37Ra
Expression system: Escherichia coli
UniProt:
  • Canonical: A5U6Z7 (Residues: 1-99; Coverage: 46%)
Gene names: MRA_3015, hupB
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 36.774Å b: 53.974Å c: 41.732Å
α: 90° β: 97.01° γ: 90°
R-values:
R R work R free
0.191 0.188 0.249
Expression system: Escherichia coli