› 4pt5

X-ray diffraction
2.59Å resolution

Crystal structure of PLpro from Middle East Respiratory Syndrome (MERS) coronavirus

Released:
DOI: 10.2210/pdb4pt5/pdb
PDB entry 4pt5 coloured by chain, front view.
PDB entry 4pt5 coloured by chain, side view.
PDB entry 4pt5 coloured by chain, top view.
Source organism: Human betacoronavirus 2c EMC/2012
Entry authors: Lei H, Santarsiero BD, Lee H, Johnson ME

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ORF1a Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 36.04 KDa
Source organism: Human betacoronavirus 2c EMC/2012
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: K4LC41 (Residues: 1482-1803; Coverage: 7%)
Gene name: orf1ab
Sequence domains: Coronavirus papain-like peptidase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
2 modified residues:
Ligand OCS 1 x OCS
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 100.82Å b: 47.26Å c: 88.77Å
α: 90° β: 121.6° γ: 90°
R-values:
R R work R free
0.232 0.229 0.299
Expression system: Escherichia coli BL21

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