› 4pwn

X-ray diffraction
1.84Å resolution

Crystal structure of Active WNK1 kinase

Released:
DOI: 10.2210/pdb4pwn/pdb
PDB entry 4pwn coloured by chain, front view.
PDB entry 4pwn coloured by chain, side view.
PDB entry 4pwn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Chloride sensing by WNK1 involves inhibition of autophosphorylation.
Piala AT, Moon TM, Akella R, He H, Cobb MH, Goldsmith EJ
Sci Signal 7 ra41 (2014)
PMID: 24803536

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase WNK1 Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H4A3 (Residues: 210-374, 375-482; Coverage: 12%)
Gene names: HSN2, KDP, KIAA0344, PRKWNK1, WNK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 44.737Å b: 64.226Å c: 42.082Å
α: 90° β: 92° γ: 90°
R-values:
R R work R free
0.231 0.229 0.274
Expression system: Escherichia coli

Quick links

Citations

77 review citations

Cation-chloride cotransporters in neuronal development, plasticity and disease.
Kaila et al. (2014)
76 more

2 mentions without citation

Chloride sensing by WNK1 involves inhibition of autophosphorylation.
Piala et al. (2014)
1 more