› 4pxd

X-ray diffraction
2.2Å resolution

The crystal structure of EcAAH in complex with allantoate

Released:
DOI: 10.2210/pdb4pxd/pdb
PDB entry 4pxd coloured by chain, front view.
PDB entry 4pxd coloured by chain, side view.
PDB entry 4pxd coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural insights into the substrate specificity of (s)-ureidoglycolate amidohydrolase and its comparison with allantoate amidohydrolase.
Shin I, Han K, Rhee S
J Mol Biol 426 3028-40 (2014)
PMID: 25020232

Function and Biology Details

Reaction catalysed: 
Allantoate + H(2)O = (S)-ureidoglycine + NH(3) + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Allantoate amidohydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 413 amino acids
Theoretical weight: 45.88 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P77425 (Residues: 1-411; Coverage: 100%)
Gene names: JW0504, allC, b0516, glxB7, ylbB
Sequence domains: Peptidase family M20/M25/M40
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand 1AL 2 x 1AL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21212
Unit cell:
a: 106.829Å b: 171.864Å c: 43.244Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.218
Expression system: Escherichia coli

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Citations

1 review citation

Transport, functions, and interaction of calcium and manganese in plant organellar compartments.
He et al. (2021)
3 other articles

2 mentions without citation

Structural insights into enzymatic [4+2] aza-cycloaddition in thiopeptide antibiotic biosynthesis.
Cogan et al. (2017)
1 more