› 4pxi

X-ray diffraction
3.2Å resolution

Elucidation of the Structural and Functional Mechanism of Action of the TetR Family Protein, CprB from S. coelicolor A3(2)

Released:
DOI: 10.2210/pdb4pxi/pdb
PDB entry 4pxi coloured by chain, front view.
PDB entry 4pxi coloured by chain, side view.
PDB entry 4pxi coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional basis of transcriptional regulation by TetR family protein CprB from S. coelicolor A3(2).
Bhukya H, Bhujbalrao R, Bitra A, Anand R
Nucleic Acids Res 42 10122-33 (2014)
PMID: 25092919

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117993 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
CprB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 215 amino acids
Theoretical weight: 23.72 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: O66122 (Residues: 1-215; Coverage: 100%)
Gene name: cprB
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2
DNA (5'-D(*AP*CP*AP*TP*AP*CP*GP*GP*GP*AP*CP*GP*CP*CP*CP*CP*GP*TP*TP*TP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 22 nucleotides
Theoretical weight: 6.71 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*TP*AP*AP*AP*CP*GP*GP*GP*GP*CP*GP*TP*CP*CP*CP*GP*TP*AP*TP*GP*T)-3') Chain: F
Molecule details ›
Chain: F
Length: 22 nucleotides
Theoretical weight: 6.79 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P32
Unit cell:
a: 149.06Å b: 149.06Å c: 69.07Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.22 0.294
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Specialised metabolites regulating antibiotic biosynthesis in Streptomyces spp.
Niu et al. (2016)
7 more

3 mentions without citation

Structural and functional basis of transcriptional regulation by TetR family protein CprB from S. coelicolor A3(2).
Bhukya et al. (2014)
2 more