› 4pxj

X-ray diffraction
2.06Å resolution

Crystallographic structure of the LZII fragment (anti-parallel orientation) from JIP3

Released:
DOI: 10.2210/pdb4pxj/pdb
PDB entry 4pxj coloured by chain, front view.
PDB entry 4pxj coloured by chain, side view.
PDB entry 4pxj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a truncated form of leucine zipper II of JIP3 reveals an unexpected antiparallel coiled-coil arrangement.
Llinas P, Chenon M, Nguyen TQ, Moreira C, de Régibus A, Coquard A, Ramos MJ, Guérois R, Fernandes PA, Ménétrey J
Acta Crystallogr F Struct Biol Commun 72 198-206 (2016)
PMID: 26919523

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-Jun-amino-terminal kinase-interacting protein 3 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 61 amino acids
Theoretical weight: 6.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UPT6 (Residues: 433-486; Coverage: 4%)
Gene names: JIP3, KIAA1066, MAPK8IP3
Sequence domains: JNK-interacting protein leucine zipper II

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand EDO 4 x EDO
Ligand GAI 2 x GAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 137.826Å b: 63.04Å c: 50.219Å
α: 90° β: 97.95° γ: 90°
R-values:
R R work R free
0.18 0.18 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Structural characterization of the RH1-LZI tandem of JIP3/4 highlights RH1 domains as a cytoskeletal motor-binding motif.
Vilela et al. (2019)
2 more

1 mention without citation

Structure of a truncated form of leucine zipper II of JIP3 reveals an unexpected antiparallel coiled-coil arrangement.
Llinas et al. (2016)