› 4pxk

X-ray diffraction
2.5Å resolution

Crystal structure of Haloarcula marismortui bacteriorhodopsin I D94N mutant

Released:
DOI: 10.2210/pdb4pxk/pdb
PDB entry 4pxk coloured by chain, front view.
PDB entry 4pxk coloured by chain, side view.
PDB entry 4pxk coloured by chain, top view.
Source organism: Haloarcula marismortui ATCC 43049
Primary publication:
Crystal structure of Escherichia coli-expressed Haloarcula marismortui bacteriorhodopsin I in the trimeric form.
Shevchenko V, Gushchin I, Polovinkin V, Round E, Borshchevskiy V, Utrobin P, Popov A, Balandin T, Büldt G, Gordeliy V
PLoS One 9 e112873 (2014)
PMID: 25479443

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin-I Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.03 KDa
Source organism: Haloarcula marismortui ATCC 43049
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5UXY6 (Residues: 1-250; Coverage: 100%)
Gene names: bop, rrnAC3161
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand RET 1 x RET
Ligand LFA 19 x LFA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P321
Unit cell:
a: 102.854Å b: 102.854Å c: 60.003Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.192 0.237
Expression system: Escherichia coli

Quick links

Citations

1 review citation

ESR - a retinal protein with unusual properties from Exiguobacterium sibiricum.
Petrovskaya et al. (2015)
6 other articles

5 mentions without citation

A Comparative Study of Modern Homology Modeling Algorithms for Rhodopsin Structure Prediction.
Nikolaev et al. (2018)
4 more