› 4pzk

X-ray diffraction
1.5Å resolution

Crystal strucrure of putative RNA methyltransferase from Bacillus anthracis.

Released:
DOI: 10.2210/pdb4pzk/pdb
PDB entry 4pzk coloured by chain, front view.
PDB entry 4pzk coloured by chain, side view.
PDB entry 4pzk coloured by chain, top view.
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Entry authors: Maltseva N, Kim Y, Shatsman S, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105713 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative tRNA (cytidine(34)-2'-O)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.77 KDa
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A6L7HQJ3 (Residues: 1-162; Coverage: 100%)
Gene name: GBAA_0547
Sequence domains: SpoU rRNA Methylase family
Structure domains: Alpha/beta knot

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 48.249Å b: 59.758Å c: 111.455Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.145 0.185
Expression system: Escherichia coli BL21

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