› 4q0k

X-ray diffraction
1.34Å resolution

Crystal Structure of Phytohormone Binding Protein from Medicago truncatula in complex with gibberellic acid (GA3)

Released:
DOI: 10.2210/pdb4q0k/pdb
PDB entry 4q0k coloured by chain, front view.
PDB entry 4q0k coloured by chain, side view.
PDB entry 4q0k coloured by chain, top view.
Source organism: Medicago truncatula
Primary publication:
Specific binding of gibberellic acid by cytokinin-specific binding proteins: a new aspect of plant hormone-binding proteins with the PR-10 fold.
Ruszkowski M, Sliwiak J, Ciesielska A, Barciszewski J, Sikorski M, Jaskolski M
Acta Crystallogr D Biol Crystallogr 70 2032-41 (2014)
PMID: 25004979

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytohormone-binding protein Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.02 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli
UniProt:
  • Canonical: G7J032 (Residues: 1-156; Coverage: 100%)
Gene name: MTR_3g055120
Sequence domains: Pathogenesis-related protein Bet v 1 family
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

2 bound ligands:
Ligand GA3 1 x GA3
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P65
Unit cell:
a: 55.8Å b: 55.8Å c: 99.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.124 0.123 0.157
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Ligand Binding of PR-10 Proteins with a Particular Focus on the Bet v 1 Allergen Family.
Aglas et al. (2020)
1 more

1 mention without citation

ANS complex of St John's wort PR-10 protein with 28 copies in the asymmetric unit: a fiendish combination of pseudosymmetry with tetartohedral twinning.
Sliwiak et al. (2015)