› 4q0w

X-ray diffraction
2.1Å resolution

he catalytic core of Rad2 in complex with DNA substrate (complex II)

Released:
DOI: 10.2210/pdb4q0w/pdb
PDB entry 4q0w coloured by chain, front view.
PDB entry 4q0w coloured by chain, side view.
PDB entry 4q0w coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structure of the catalytic core of Rad2: insights into the mechanism of substrate binding.
Miętus M, Nowak E, Jaciuk M, Kustosz P, Studnicka J, Nowotny M
Nucleic Acids Res 42 10762-75 (2014)
PMID: 25120270

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118000 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA repair protein RAD2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 42.19 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07276 (Residues: 2-986; Coverage: 35%)
Gene names: RAD2, YGR258C
Sequence domains:
DNA (5'-D(*TP*TP*TP*GP*AP*TP*CP*CP*GP*TP*CP*CP*AP*CP*CP*TP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.41 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
DNA (5'-D(*TP*TP*AP*GP*GP*TP*GP*GP*AP*CP*GP*GP*AP*TP*CP*AP*TP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.59 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21212
Unit cell:
a: 126.641Å b: 76.813Å c: 108.192Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.23
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

XPA: A key scaffold for human nucleotide excision repair.
Sugitani et al. (2016)
3 more

3 mentions without citation

Classification of proteins with shared motifs and internal repeats in the ECOD database.
Schaeffer et al. (2016)
2 more