› 4q2c

X-ray diffraction
2.5Å resolution

Crystal structure of CRISPR-associated protein

Released:
DOI: 10.2210/pdb4q2c/pdb
PDB entry 4q2c coloured by chain, front view.
PDB entry 4q2c coloured by chain, side view.
PDB entry 4q2c coloured by chain, top view.
Source organism: Thermobaculum terrenum ATCC BAA-798
Primary publication:
Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3.
Gong B, Shin M, Sun J, Jung CH, Bolt EL, van der Oost J, Kim JS
Proc Natl Acad Sci U S A 111 16359-64 (2014)
PMID: 25368186

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112293 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HD Cas3-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 949 amino acids
Theoretical weight: 105.15 KDa
Source organism: Thermobaculum terrenum ATCC BAA-798
Expression system: Escherichia coli
UniProt:
  • Canonical: D1CGD0 (Residues: 1-944; Coverage: 100%)
Gene name: Tter_1895
Sequence domains:
Structure domains: Hypothetical protein af1432

Ligands and Environments

1 bound ligand:
Ligand NI 3 x NI
1 modified residue:
Ligand MSE 21 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2221
Unit cell:
a: 104.268Å b: 214.558Å c: 102.196Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.229
Expression system: Escherichia coli

Quick links

Citations

19 review citations

An updated evolutionary classification of CRISPR-Cas systems.
Makarova et al. (2015)
18 more

4 mentions without citation

Cas3 Protein-A Review of a Multi-Tasking Machine.
He et al. (2020)
3 more