› 4q2e

X-ray diffraction
3.4Å resolution

CRYSTAL STRUCTURE OF AN INTRAMEMBRANE CDP-DAG SYNTHETASE CENTRAL FOR PHOSPHOLIPID BIOSYNTHESIS (S200C/S258C, active mutant)

Released:
DOI: 10.2210/pdb4q2e/pdb
PDB entry 4q2e coloured by chain, front view.
PDB entry 4q2e coloured by chain, side view.
PDB entry 4q2e coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Structure and mechanism of an intramembrane liponucleotide synthetase central for phospholipid biosynthesis.
Liu X, Yin Y, Wu J, Liu Z
Nat Commun 5 4244 (2014)
PMID: 24968740

Function and Biology Details

Reaction catalysed: 
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194783 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidate cytidylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 32.33 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1B7 (Residues: 1-270; Coverage: 100%)
Gene names: TM_1397, cdsA
Sequence domains: Cytidylyltransferase family

Ligands and Environments

3 bound ligands:
Ligand HG 6 x HG
Ligand MG 2 x MG
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P6522
Unit cell:
a: 142.08Å b: 142.08Å c: 198.373Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.299 0.299 0.338
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Reprogramming of fatty acid metabolism in cancer.
Koundouros et al. (2020)
5 more

8 mentions without citation

Lipid synthesis and transport are coupled to regulate membrane lipid dynamics in the endoplasmic reticulum.
Balla et al. (2020)
7 more