› 4q35

X-ray diffraction
2.39Å resolution

Structure of a membrane protein

Released:
DOI: 10.2210/pdb4q35/pdb
PDB entry 4q35 coloured by chain, front view.
PDB entry 4q35 coloured by chain, side view.
PDB entry 4q35 coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
Structural basis for lipopolysaccharide insertion in the bacterial outer membrane.
Qiao S, Luo Q, Zhao Y, Zhang XC, Huang Y
Nature 511 108-11 (2014)
PMID: 24990751

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182951 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
LPS-assembly protein LptD Chain: A
Molecule details ›
Chain: A
Length: 802 amino acids
Theoretical weight: 91.64 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83SQ0 (Residues: 1-784; Coverage: 100%)
Gene names: S0053, SF0051, imp, lptD, ostA
Sequence domains:
LPS-assembly lipoprotein LptE Chain: B
Molecule details ›
Chain: B
Length: 175 amino acids
Theoretical weight: 19.51 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83LX4 (Residues: 19-193; Coverage: 100%)
Gene names: S0662, SF0640, lptE, rlpB
Sequence domains: Lipopolysaccharide-assembly
Structure domains: Lipoprotein like domain

Ligands and Environments

3 bound ligands:
Ligand C8E 6 x C8E
Ligand LDA 7 x LDA
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 240.823Å b: 116.62Å c: 68.038Å
α: 90° β: 103.71° γ: 90°
R-values:
R R work R free
0.189 0.186 0.231
Expression system: Escherichia coli

Quick links

Citations

32 review citations

Outer membrane lipoprotein biogenesis: Lol is not the end.
Konovalova et al. (2015)
31 more

143 mentions without citation

Biochemistry and genetics of inherited disorders of peroxisomal fatty acid metabolism.
Van Veldhoven PP. (2010)
142 more