› 4q3l

X-ray diffraction
3.01Å resolution

Crystal structure of MGS-M2, an alpha/beta hydrolase enzyme from a Medee basin deep-sea metagenome library

Released:
DOI: 10.2210/pdb4q3l/pdb
PDB entry 4q3l coloured by chain, front view.
PDB entry 4q3l coloured by chain, side view.
PDB entry 4q3l coloured by chain, top view.
Source organism: unidentified
Primary publication:
Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats.
Alcaide M, Stogios PJ, Lafraya Á, Tchigvintsev A, Flick R, Bargiela R, Chernikova TN, Reva ON, Hai T, Leggewie CC, Katzke N, La Cono V, Matesanz R, Jebbar M, Jaeger KE, Yakimov MM, Yakunin AF, Golyshin PN, Golyshina OV, Savchenko A, Ferrer M
Environ Microbiol 17 332-45 (2015)
PMID: 25330254

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 297 amino acids
Theoretical weight: 34.06 KDa
Source organism: unidentified
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B5KBT7 (Residues: 1-276; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 105.568Å b: 139.134Å c: 111.109Å
α: 90° β: 89.94° γ: 90°
R-values:
R R work R free
0.203 0.201 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Living at the Extremes: Extremophiles and the Limits of Life in a Planetary Context.
Merino et al. (2019)
3 more

1 mention without citation

The Thaumarchaeon N. gargensis carries functional bioABD genes and has a promiscuous E. coli ΔbioH-complementing esterase EstN1.
Chow et al. (2018)