› 4q3o

X-ray diffraction
1.74Å resolution

Crystal structure of MGS-MT1, an alpha/beta hydrolase enzyme from a Lake Matapan deep-sea metagenome library

Released:
DOI: 10.2210/pdb4q3o/pdb
PDB entry 4q3o coloured by chain, front view.
PDB entry 4q3o coloured by chain, side view.
PDB entry 4q3o coloured by chain, top view.
Source organism: unidentified
Primary publication:
Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats.
Alcaide M, Stogios PJ, Lafraya Á, Tchigvintsev A, Flick R, Bargiela R, Chernikova TN, Reva ON, Hai T, Leggewie CC, Katzke N, La Cono V, Matesanz R, Jebbar M, Jaeger KE, Yakimov MM, Yakunin AF, Golyshin PN, Golyshina OV, Savchenko A, Ferrer M
Environ Microbiol 17 332-45 (2015)
PMID: 25330254

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 348 amino acids
Theoretical weight: 38.44 KDa
Source organism: unidentified
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B5KQQ5 (Residues: 1-348; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand CL 10 x CL
Ligand MES 10 x MES
Ligand GOL 17 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 189.454Å b: 131.397Å c: 112.441Å
α: 90° β: 103.55° γ: 90°
R-values:
R R work R free
0.147 0.145 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Living at the Extremes: Extremophiles and the Limits of Life in a Planetary Context.
Merino et al. (2019)
3 more

3 mentions without citation

A Novel Halotolerant Thermoalkaliphilic Esterase from Marine Bacterium Erythrobacter seohaensis SW-135.
Huo et al. (2017)
2 more