› 4q4j

X-ray diffraction
3.2Å resolution

Structure of crosslinked TM287/288_S498C_S520C mutant

Released:
DOI: 10.2210/pdb4q4j/pdb
PDB entry 4q4j coloured by chain, front view.
PDB entry 4q4j coloured by chain, side view.
PDB entry 4q4j coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter.
Hohl M, Hürlimann LM, Böhm S, Schöppe J, Grütter MG, Bordignon E, Seeger MA
Proc Natl Acad Sci U S A 111 11025-30 (2014)
PMID: 25030449

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194496 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ABC transporter, ATP-binding protein Chain: A
Molecule details ›
Chain: A
Length: 587 amino acids
Theoretical weight: 65 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYC3 (Residues: 2-577; Coverage: 100%)
Gene name: TM_0287
Sequence domains:
Structure domains:
Uncharacterized ABC transporter ATP-binding protein TM_0288 Chain: B
Molecule details ›
Chain: B
Length: 598 amino acids
Theoretical weight: 67.81 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYC4 (Residues: 1-598; Coverage: 100%)
Gene name: TM_0288
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 216.67Å b: 84.14Å c: 113.46Å
α: 90° β: 93.32° γ: 90°
R-values:
R R work R free
0.239 0.237 0.287
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Mechanistic diversity in ATP-binding cassette (ABC) transporters.
Locher KP. (2016)
10 more

7 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
6 more