› 4q4n

X-ray diffraction
1.38Å resolution

Structure of the Resuscitation Promoting Factor Interacting protein RipA mutated at H432

Released:
DOI: 10.2210/pdb4q4n/pdb
PDB entry 4q4n coloured by chain, front view.
PDB entry 4q4n coloured by chain, side view.
PDB entry 4q4n coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Mutational and structural study of RipA, a key enzyme in Mycobacterium tuberculosis cell division: evidence for the L-to-D inversion of configuration of the catalytic cysteine.
Squeglia F, Ruggiero A, Romano M, Vitagliano L, Berisio R
Acta Crystallogr D Biol Crystallogr 70 2295-300 (2014)
PMID: 25195744

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidoglycan endopeptidase RipA Chain: A
Molecule details ›
Chain: A
Length: 472 amino acids
Theoretical weight: 49.79 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: O53168 (Residues: 1-472; Coverage: 100%)
Gene names: Rv1477, ripA
Sequence domains: NlpC/P60 family
Structure domains: endopeptidase domain like (from Nostoc punctiforme)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 36.702Å b: 65.492Å c: 68.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.186
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Chemistry of Peptidoglycan in Mycobacterium tuberculosis Life Cycle: An off-the-wall Balance of Synthesis and Degradation.
Squeglia et al. (2018)
2 more

2 mentions without citation

The Cell Wall Hydrolytic NlpC/P60 Endopeptidases in Mycobacterial Cytokinesis: A Structural Perspective.
Squeglia et al. (2019)
1 more