We've built a new version of this page with an interactive 3D viewer. Try it out in beta and help us improve:

› 4q71

X-ray diffraction
2.2Å resolution

Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (PutA) Mutant D779W

Released:
DOI: 10.2210/pdb4q71/pdb
Model geometry
Fit model/data
PDB entry 4q71 coloured by chain, front view.
PDB entry 4q71 coloured by chain, side view.
PDB entry 4q71 coloured by chain, top view.
Source organism: Bradyrhizobium diazoefficiens USDA 110
Primary publication:
Kinetic and structural characterization of tunnel-perturbing mutants in Bradyrhizobium japonicum proline utilization A.
Arentson BW, Luo M, Pemberton TA, Tanner JJ, Becker DF
OpenAccess logo Biochemistry 53 5150-61 (2014)
PMID: 25046425

Function and Biology Details

Reactions catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+).
L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein PutA Chains: A, B
Molecule details ›
Chains: A, B
Length: 1001 amino acids
Theoretical weight: 107.77 KDa
Source organism: Bradyrhizobium diazoefficiens USDA 110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q89E26 (Residues: 1-999; Coverage: 100%)
Gene name: putA
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand SO4 6 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: C2
Unit cell:
a: 166.88Å b: 195.31Å c: 108.79Å
α: 90° β: 121.59° γ: 90°
R-values:
R R work R free
0.21 0.208 0.241
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Role of Proline in Pathogen and Host Interactions.
Christgen et al. (2019)
1 more

2 mentions without citation

Structure, function, and mechanism of proline utilization A (PutA).
Liu et al. (2017)
1 more