› 4q76

X-ray diffraction
1.9Å resolution

Crystal structure of Nfs2 C384S mutant, the plastidial cysteine desulfurase from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb4q76/pdb
PDB entry 4q76 coloured by chain, front view.
PDB entry 4q76 coloured by chain, side view.
PDB entry 4q76 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana.
Roret T, Pégeot H, Couturier J, Mulliert G, Rouhier N, Didierjean C
Acta Crystallogr F Struct Biol Commun 70 1180-5 (2014)
PMID: 25195888

Function and Biology Details

Reactions catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase 1, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 429 amino acids
Theoretical weight: 47.16 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93WX6 (Residues: 36-463; Coverage: 92%)
Gene names: At1g08490, CpNIFS, CpNIFS1, NFS2, T27G7.17
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 81.718Å b: 68.387Å c: 87.58Å
α: 90° β: 94.84° γ: 90°
R-values:
R R work R free
0.235 0.233 0.262
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Roles and maturation of iron-sulfur proteins in plastids.
Przybyla-Toscano et al. (2018)
2 more

2 mentions without citation

Snapshots of C-S Cleavage in Egt2 Reveals Substrate Specificity and Reaction Mechanism.
Irani et al. (2018)
1 more