› 4q7y

X-ray diffraction
2.7Å resolution

Neutrophil serine protease 4 (PRSS57) apo form 2

Released:
DOI: 10.2210/pdb4q7y/pdb
PDB entry 4q7y coloured by chain, front view.
PDB entry 4q7y coloured by chain, side view.
PDB entry 4q7y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of neutrophil serine protease 4 reveal an unusual mechanism of substrate recognition by a trypsin-fold protease.
Lin SJ, Dong KC, Eigenbrot C, van Lookeren Campagne M, Kirchhofer D
Structure 22 1333-1340 (2014)
PMID: 25156428

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Serine protease 57 Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 27 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6UWY2 (Residues: 34-283; Coverage: 99%)
Gene names: PRSS57, PRSSL1, UNQ782/PRO1599
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P63
Unit cell:
a: 86.636Å b: 86.636Å c: 69.351Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.187 0.248
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Surface loops of trypsin-like serine proteases as determinants of function.
Goettig et al. (2019)
3 more

1 mention without citation

Effects of Glycosylation on the Enzymatic Activity and Mechanisms of Proteases.
Goettig P. (2016)