› 4qdi

X-ray diffraction
1.8Å resolution

Crystal structure II of MurF from Acinetobacter baumannii

Released:
DOI: 10.2210/pdb4qdi/pdb
PDB entry 4qdi coloured by chain, front view.
PDB entry 4qdi coloured by chain, side view.
PDB entry 4qdi coloured by chain, top view.
Source organism: Acinetobacter baumannii AB307-0294
Primary publication:
ATP-binding mode including a carbamoylated lysine and two Mg(2+) ions, and substrate-binding mode in Acinetobacter baumannii MurF.
Cha SS, An YJ, Jeong CS, Yu JH, Chung KM
Biochem Biophys Res Commun 450 1045-50 (2014)
PMID: 24978312

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102250 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase Chain: A
Molecule details ›
Chain: A
Length: 472 amino acids
Theoretical weight: 51.71 KDa
Source organism: Acinetobacter baumannii AB307-0294
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0J9X1Z8 (Residues: 1-466; Coverage: 100%)
Gene names: ABBFA_000315, ABBFA_00314, murF
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ATP 1 x ATP
Ligand UDP 1 x UDP
Ligand MG 1 x MG
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P3221
Unit cell:
a: 85.783Å b: 85.783Å c: 130.203Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.227 0.253
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structure of the essential peptidoglycan amidotransferase MurT/GatD complex from Streptococcus pneumoniae.
Morlot et al. (2018)

5 mentions without citation

Structural basis of cell wall peptidoglycan amidation by the GatD/MurT complex of Staphylococcus aureus.
Nöldeke et al. (2018)
4 more