› 4qdl

X-ray diffraction
2.7Å resolution

Crystal structure of E.coli Cas1-Cas2 complex

Released:
DOI: 10.2210/pdb4qdl/pdb
Model geometry
Fit model/data
PDB entry 4qdl coloured by chain, front view.
PDB entry 4qdl coloured by chain, side view.
PDB entry 4qdl coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Tamulaitiene G, Sinkunas T, Silanskas A, Gasiunas G, Grazulis S, Siksnys V

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155374 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CRISPR-associated endonuclease Cas1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 305 amino acids
Theoretical weight: 33.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q46896 (Residues: 1-305; Coverage: 100%)
Gene names: JW2725, b2755, cas1, ygbT
Sequence domains: CRISPR associated protein Cas1
Structure domains:
CRISPR-associated endoribonuclease Cas2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 104 amino acids
Theoretical weight: 11.74 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P45956 (Residues: 1-94; Coverage: 100%)
Gene names: JW5438, b2754, cas2, ygbF
Sequence domains: CRISPR-associated protein (Cas_Cas2CT1978)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 92.764Å b: 127.402Å c: 96.64Å
α: 90° β: 100.89° γ: 90°
R-values:
R R work R free
0.23 0.227 0.261
Expression system: Escherichia coli BL21

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