4qek

X-ray diffraction
1.3Å resolution

Crystal structure of Antigen 85C-S124A mutant

Released:

Function and Biology Details

Reactions catalysed:
2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-162087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diacylglycerol acyltransferase/mycolyltransferase Ag85C Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 32.92 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQN9 (Residues: 46-340; Coverage: 100%)
Gene names: MTCI5.03c, Rv0129c, fbpC, mpt45
Sequence domains: Putative esterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 136.736Å b: 67.859Å c: 35.692Å
α: 90° β: 94.36° γ: 90°
R-values:
R R work R free
0.127 0.127 0.143
Expression system: Escherichia coli