› 4qg3

X-ray diffraction
2Å resolution

Crystal structure of mutant ribosomal protein G219V TthL1 in complex with 80nt 23S RNA from Thermus thermophilus

Released:
DOI: 10.2210/pdb4qg3/pdb
PDB entry 4qg3 coloured by chain, front view.
PDB entry 4qg3 coloured by chain, side view.
PDB entry 4qg3 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Protein-RNA affinity of ribosomal protein L1 mutants does not correlate with the number of intermolecular interactions.
Tishchenko S, Kostareva O, Gabdulkhakov A, Mikhaylina A, Nikonova E, Nevskaya N, Sarskikh A, Piendl W, Garber M, Nikonov S
Acta Crystallogr D Biol Crystallogr 71 376-86 (2015)
PMID: 25664749

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150938 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Large ribosomal subunit protein uL1 Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 24.78 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P27150 (Residues: 2-229; Coverage: 100%)
Gene names: rpl1, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Structure domains:
fragment of 23S rRNA Chain: B
Molecule details ›
Chain: B
Length: 80 nucleotides
Theoretical weight: 25.97 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
Sequence domains: Bacterial large subunit ribosomal RNA

Ligands and Environments

4 bound ligands:
Ligand SO4 5 x SO4
Ligand IPA 8 x IPA
Ligand BME 4 x BME
Ligand URE 1 x URE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 72.773Å b: 76.489Å c: 86.254Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.2 0.226
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

A unified dinucleotide alphabet describing both RNA and DNA structures.
Černý et al. (2020)
3 more

1 mention without citation

A Structure-Based Mechanism for the Denaturing Action of Urea, Guanidinium Ion and Thiocyanate Ion.
Paladino et al. (2022)