› 4qgu

X-ray diffraction
2.54Å resolution

protein domain complex with ssDNA

Released:
DOI: 10.2210/pdb4qgu/pdb
PDB entry 4qgu coloured by chain, front view.
PDB entry 4qgu coloured by chain, side view.
PDB entry 4qgu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16.
Ni X, Ru H, Ma F, Zhao L, Shaw N, Feng Y, Ding W, Gong W, Wang Q, Ouyang S, Cheng G, Liu ZJ
J Mol Cell Biol 8 51-61 (2016)
PMID: 26246511

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118016 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Gamma-interferon-inducible protein 16 Chains: A, B
Molecule details ›
Chains: A, B
Length: 206 amino acids
Theoretical weight: 23.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16666 (Residues: 192-393; Coverage: 26%)
Gene names: IFI16, IFNGIP1
Sequence domains: HIN-200/IF120x domain
Structure domains: Nucleic acid-binding proteins
DNA (5'-D(P*AP*GP*GP*CP*CP*GP*GP*CP*GP*TP*GP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 nucleotides
Theoretical weight: 3.73 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21212
Unit cell:
a: 85.715Å b: 91.865Å c: 69.978Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.238 0.258
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Innate Sensing of DNA Virus Genomes.
Ma et al. (2018)
11 more

4 mentions without citation

Activation and Immune Regulation Mechanisms of PYHIN Family During Microbial Infection.
Fan et al. (2021)
3 more