› 4qgv

X-ray diffraction
1.73Å resolution

Crystal structure of the R132K:R111L mutant of Cellular Retinoic Acid Binding ProteinII complexed with a synthetic ligand (Merocyanine) at 1.73 angstrom resolution.

Released:
DOI: 10.2210/pdb4qgv/pdb
PDB entry 4qgv coloured by chain, front view.
PDB entry 4qgv coloured by chain, side view.
PDB entry 4qgv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
"Turn-on" protein fluorescence: in situ formation of cyanine dyes.
Yapici I, Lee KS, Berbasova T, Nosrati M, Jia X, Vasileiou C, Wang W, Santos EM, Geiger JH, Borhan B
J Am Chem Soc 137 1073-80 (2015)
PMID: 25534273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-151430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular retinoic acid-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 137 amino acids
Theoretical weight: 15.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29373 (Residues: 2-138; Coverage: 99%)
Gene name: CRABP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand LMC 1 x LMC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P1
Unit cell:
a: 34.785Å b: 37.34Å c: 60.091Å
α: 104.53° β: 106.04° γ: 90.87°
R-values:
R R work R free
0.199 0.197 0.24
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Cyanine polyene reactivity: scope and biomedical applications.
Gorka et al. (2015)
8 more

1 mention without citation

"Turn-on" protein fluorescence: in situ formation of cyanine dyes.
Yapici et al. (2015)