› 4qh0

X-ray diffraction
2Å resolution

Crystal structure of NucA from Streptococcus agalactiae with magnesium ion bound

Released:
DOI: 10.2210/pdb4qh0/pdb
PDB entry 4qh0 coloured by chain, front view.
PDB entry 4qh0 coloured by chain, side view.
PDB entry 4qh0 coloured by chain, top view.
Source organism: Streptococcus agalactiae ILRI112
Primary publication:
Structural characterization of the virulence factor nuclease A from Streptococcus agalactiae.
Moon AF, Gaudu P, Pedersen LC
Acta Crystallogr D Biol Crystallogr 70 2937-49 (2014)
PMID: 25372684

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-165097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-entry nuclease (Competence-specific nuclease) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 225 amino acids
Theoretical weight: 25.24 KDa
Source organism: Streptococcus agalactiae ILRI112
Expression system: Escherichia coli
Structure domains: Extracellular Endonuclease, subunit A

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand SO4 23 x SO4
Ligand CL 6 x CL
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P63
Unit cell:
a: 123.721Å b: 123.721Å c: 157.403Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.155 0.19
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Pathogen-Derived Nucleases: An Effective Weapon for Escaping Extracellular Traps.
Liao et al. (2022)
3 other articles

3 mentions without citation

Lessons from making the Structural Classification of Proteins (SCOP) and their implications for protein structure modelling.
Andreeva A. (2016)
2 more