› 4qi1

X-ray diffraction
1.85Å resolution

Crystal structure of H. walsbyi bacteriorhodopsin

Released:
DOI: 10.2210/pdb4qi1/pdb
PDB entry 4qi1 coloured by chain, front view.
PDB entry 4qi1 coloured by chain, side view.
PDB entry 4qi1 coloured by chain, top view.
Source organism: Haloquadratum walsbyi DSM 16790
Primary publication:
Structural and Functional Studies of a Newly Grouped Haloquadratum walsbyi Bacteriorhodopsin Reveal the Acid-resistant Light-driven Proton Pumping Activity.
Hsu MF, Fu HY, Cai CJ, Yi HP, Yang CS, Wang AH
J Biol Chem 290 29567-77 (2015)
PMID: 26483542

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172747 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin-I Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 262 amino acids
Theoretical weight: 28.49 KDa
Source organism: Haloquadratum walsbyi DSM 16790
Expression system: Escherichia coli
UniProt:
  • Canonical: Q18DH8 (Residues: 3-254; Coverage: 99%)
Gene names: HQ_1014A, bop1, bopI
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand RET 3 x RET
Ligand MPG 9 x MPG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: C2
Unit cell:
a: 106.697Å b: 61.634Å c: 119.512Å
α: 90° β: 116.17° γ: 90°
R-values:
R R work R free
0.199 0.197 0.237
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

A Unique Light-Driven Proton Transportation Signal in Halorhodopsin from Natronomonas pharaonis.
Chen et al. (2016)
4 more

4 mentions without citation

NeoR, a near-infrared absorbing rhodopsin.
Broser et al. (2020)
3 more