› 4qil

X-ray diffraction
2.9Å resolution

Crystal structure of the ROQ domain of human Roquin in complex with the Hmg19 stem-loop RNA

Released:
DOI: 10.2210/pdb4qil/pdb
PDB entry 4qil coloured by chain, front view.
PDB entry 4qil coloured by chain, side view.
PDB entry 4qil coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The ROQ domain of Roquin recognizes mRNA constitutive-decay element and double-stranded RNA.
Tan D, Zhou M, Kiledjian M, Tong L
Nat Struct Mol Biol 21 679-85 (2014)
PMID: 25026078

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178323 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Roquin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5TC82 (Residues: 88-407; Coverage: 28%)
Gene names: KIAA2025, RC3H1, RNF198
Sequence domains:
5'-R(*CP*UP*CP*CP*CP*UP*UP*CP*UP*GP*UP*GP*AP*AP*GP*GP*GP*GP*A)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 19 nucleotides
Theoretical weight: 6.07 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 49.6Å b: 170Å c: 51.1Å
α: 90° β: 97° γ: 90°
R-values:
R R work R free
0.198 0.195 0.251
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

19 review citations

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
18 more

6 mentions without citation

The ROQ domain of Roquin recognizes mRNA constitutive-decay element and double-stranded RNA.
Tan et al. (2014)
5 more