› 4qiu

X-ray diffraction
1.4Å resolution

Crystal structure of Nitroalkane Oxidase from Pseudomonas aeruginosa in Mutant complex form

Released:
DOI: 10.2210/pdb4qiu/pdb
Model geometry
Fit model/data
PDB entry 4qiu coloured by chain, front view.
PDB entry 4qiu coloured by chain, side view.
PDB entry 4qiu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO581
Entry authors: Chi YM, Lee JH

Function and Biology Details

Reaction catalysed: 
A nitroalkane + H(2)O + O(2) = an aldehyde or ketone + nitrite + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitronate monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 356 amino acids
Theoretical weight: 37.48 KDa
Source organism: Pseudomonas aeruginosa PAO581
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M3KKW2 (Residues: 1-332; Coverage: 100%)
Gene name: M801_4200
Sequence domains: Nitronate monooxygenase
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
1 bound ligand:
Ligand N1P 2 x N1P
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 70.066Å b: 56.794Å c: 87.237Å
α: 90° β: 97.09° γ: 90°
R-values:
R R work R free
0.176 0.175 0.198
Expression system: Escherichia coli

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