› 4qma

X-ray diffraction
1.65Å resolution

Crystal Structure of a Putative Cysteine Dioxygnase From Ralstonia eutropha: An Alternative Modeling of 2GM6 from JCSG Target 361076

Released:
DOI: 10.2210/pdb4qma/pdb
PDB entry 4qma coloured by chain, front view.
PDB entry 4qma coloured by chain, side view.
PDB entry 4qma coloured by chain, top view.
Source organism: Cupriavidus pinatubonensis JMP134
Primary publication:
Structures of Arg- and Gln-type bacterial cysteine dioxygenase homologs.
Driggers CM, Hartman SJ, Karplus PA
Protein Sci 24 154-61 (2015)
PMID: 25307852

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase type I Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.01 KDa
Source organism: Cupriavidus pinatubonensis JMP134
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46R41 (Residues: 1-207; Coverage: 100%)
Gene name: Reut_B5045
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand FE 1 x FE
Ligand OXY 1 x OXY
Ligand SO4 2 x SO4
Ligand EDO 9 x EDO
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P41212
Unit cell:
a: 57.007Å b: 57.007Å c: 216.683Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.2
Expression system: Escherichia coli

Quick links

Citations

12 citation in other articles

Structures of Arabidopsis thaliana oxygen-sensing plant cysteine oxidases 4 and 5 enable targeted manipulation of their activity.
White et al. (2020)
11 more

5 mentions without citation

The cysteine dioxygenase homologue from Pseudomonas aeruginosa is a 3-mercaptopropionate dioxygenase.
Tchesnokov et al. (2015)
4 more