› 4qmh

X-ray diffraction
1.65Å resolution

The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array

Released:
DOI: 10.2210/pdb4qmh/pdb
PDB entry 4qmh coloured by chain, front view.
PDB entry 4qmh coloured by chain, side view.
PDB entry 4qmh coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array.
Fox JC, Howard AE, Currie JD, Rogers SL, Slep KC
Mol Biol Cell 25 2375-92 (2014)
PMID: 24966168

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194237 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein mini spindles Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 26.3 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VEZ3 (Residues: 848-1084; Coverage: 12%)
Gene names: CG5000, msps
Sequence domains: CLASP N terminal
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 31.788Å b: 32.613Å c: 59.795Å
α: 100.05° β: 95.23° γ: 109.95°
R-values:
R R work R free
0.159 0.157 0.187
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Control of microtubule organization and dynamics: two ends in the limelight.
Akhmanova et al. (2015)
5 more

2 mentions without citation

The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array.
Fox et al. (2014)
1 more