› 4qnl

X-ray diffraction
2.41Å resolution

Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C

Released:
DOI: 10.2210/pdb4qnl/pdb
PDB entry 4qnl coloured by chain, front view.
PDB entry 4qnl coloured by chain, side view.
PDB entry 4qnl coloured by chain, top view.
Source organism: Escherichia phage vB_EcoP_G7C
Primary publication:
Function of bacteriophage G7C esterase tailspike in host cell adsorption.
Prokhorov NS, Riccio C, Zdorovenko EL, Shneider MM, Browning C, Knirel YA, Leiman PG, Letarov AV
Mol Microbiol 105 385-398 (2017)
PMID: 28513100

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124145 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tail fiber protein Chain: A
Molecule details ›
Chain: A
Length: 859 amino acids
Theoretical weight: 94.84 KDa
Source organism: Escherichia phage vB_EcoP_G7C
Expression system: Escherichia coli
UniProt:
  • Canonical: G0XNW5 (Residues: 1-851; Coverage: 100%)
Gene name: gp63.1
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand PEG 3 x PEG
Ligand EDO 2 x EDO
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
Ligand CL 4 x CL
1 modified residue:
Ligand MSE 23 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R32
Unit cell:
a: 92.773Å b: 92.773Å c: 551.926Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.159 0.221
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Reprogramming bacteriophage host range: design principles and strategies for engineering receptor binding proteins.
Dunne et al. (2021)
6 more

2 mentions without citation

Klebsiella phage KP34gp57 capsular depolymerase structure and function: from a serendipitous finding to the design of active mini-enzymes against K. pneumoniae.
Maciejewska et al. (2023)
1 more