› 4qnu

X-ray diffraction
2.6Å resolution

Crystal structure of CmoB bound with Cx-SAM in P21212

Released:
DOI: 10.2210/pdb4qnu/pdb
PDB entry 4qnu coloured by chain, front view.
PDB entry 4qnu coloured by chain, side view.
PDB entry 4qnu coloured by chain, top view.
Source organism: Escherichia coli APEC O1
Primary publication:
Determinants of the CmoB carboxymethyl transferase utilized for selective tRNA wobble modification.
Kim J, Xiao H, Koh J, Wang Y, Bonanno JB, Thomas K, Babbitt PC, Brown S, Lee YS, Almo SC
Nucleic Acids Res 43 4602-13 (2015)
PMID: 25855808

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA U34 carboxymethyltransferase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 323 amino acids
Theoretical weight: 37.05 KDa
Source organism: Escherichia coli APEC O1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1AC32 (Residues: 1-323; Coverage: 100%)
Gene names: APECO1_921, Ecok1_17280, cmoB
Sequence domains: Protein of unknown function (DUF1698)

Ligands and Environments

2 bound ligands:
Ligand GEK 8 x GEK
Ligand PO4 10 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 184.803Å b: 347.551Å c: 59.415Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Recent advances in methyltransferase biocatalysis.
Bennett et al. (2017)
2 more

3 mentions without citation

Determinants of the CmoB carboxymethyl transferase utilized for selective tRNA wobble modification.
Kim et al. (2015)
2 more