› 4qo2

X-ray diffraction
2.1Å resolution

Crystal structure of rhomboid intramembrane protease GlpG in complex with peptide derived inhibitor Ac-IATA-cmk

Released:
DOI: 10.2210/pdb4qo2/pdb
PDB entry 4qo2 coloured by chain, front view.
PDB entry 4qo2 coloured by chain, side view.
PDB entry 4qo2 coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. MC4100
Primary publication:
Substrate binding and specificity of rhomboid intramembrane protease revealed by substrate-peptide complex structures.
Zoll S, Stanchev S, Began J, Skerle J, Lepšík M, Peclinovská L, Majer P, Strisovsky K
EMBO J 33 2408-21 (2014)
PMID: 25216680

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165112 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rhomboid protease GlpG Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.74 KDa
Source organism: Escherichia coli str. K-12 substr. MC4100
Expression system: Escherichia coli
Structure domains: Rhomboid-like
6-AMINO-2-METHYL-1,7-DIHYDRO-8H-IMIDAZO[4,5-G]QUINAZOLIN-8-ONE Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 433 Da
Source organism: Escherichia coli str. K-12 substr. MC4100
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand BNG 14 x BNG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P63
Unit cell:
a: 97.99Å b: 97.99Å c: 66.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.163 0.18
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Understanding intramembrane proteolysis: from protein dynamics to reaction kinetics.
Langosch et al. (2015)
6 more

3 mentions without citation

Substrate binding and specificity of rhomboid intramembrane protease revealed by substrate-peptide complex structures.
Zoll et al. (2014)
2 more