› 4qqb

X-ray diffraction
2.8Å resolution

Structural basis for the assembly of the SXL-UNR translation regulatory complex

Released:
DOI: 10.2210/pdb4qqb/pdb
PDB entry 4qqb coloured by chain, front view.
PDB entry 4qqb coloured by chain, side view.
PDB entry 4qqb coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis for the assembly of the Sxl-Unr translation regulatory complex.
Hennig J, Militti C, Popowicz GM, Wang I, Sonntag M, Geerlof A, Gabel F, Gebauer F, Sattler M
Nature 515 287-90 (2014)
PMID: 25209665

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243154 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Protein sex-lethal Chains: A, B
Molecule details ›
Chains: A, B
Length: 176 amino acids
Theoretical weight: 19.8 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P19339 (Residues: 122-294; Coverage: 49%)
Gene names: CG43770, Sx1, Sxl
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
CSD domain-containing protein Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 72 amino acids
Theoretical weight: 8.21 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VSK3 (Residues: 185-252; Coverage: 7%)
Gene names: BcDNA:LD13080, CG7015, CR32028, Dmel\CG7015, Dmel_CG7015, MRE30, UNR, Unr, dUNR, dUnr, dunr, unr
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains: Nucleic acid-binding proteins
msl2 mRNA Chains: C, P
Molecule details ›
Chains: C, P
Length: 18 nucleotides
Theoretical weight: 5.71 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 94.36Å b: 110.97Å c: 139.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.236
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Regulation by 3'-Untranslated Regions.
Mayr C. (2017)
20 more

9 mentions without citation

Promiscuity in post-transcriptional control of gene expression: Drosophila sex-lethal and its regulatory partnerships.
Moschall et al. (2017)
8 more