› 4qqz

X-ray diffraction
2.93Å resolution

Crystal structure of T. fusca Cas3-AMPPNP

Released:
DOI: 10.2210/pdb4qqz/pdb
PDB entry 4qqz coloured by chain, front view.
PDB entry 4qqz coloured by chain, side view.
PDB entry 4qqz coloured by chain, top view.
Source organism: Thermobifida fusca YX
Primary publication:
Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation.
Huo Y, Nam KH, Ding F, Lee H, Wu L, Xiao Y, Farchione MD, Zhou S, Rajashankar K, Kurinov I, Zhang R, Ke A
Nat Struct Mol Biol 21 771-7 (2014)
PMID: 25132177

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-118028 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HD Cas3-type domain-containing protein Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 964 amino acids
Theoretical weight: 106.21 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47PJ0 (Residues: 1-944; Coverage: 100%)
Gene name: Tfu_1593
Sequence domains:
Structure domains: Hypothetical protein af1432
DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 12 nucleotides
Theoretical weight: 3.71 KDa
Source organism: Thermobifida fusca YX
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand FE 8 x FE
Ligand ANP 4 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C, CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 86.917Å b: 222.064Å c: 124.898Å
α: 90° β: 104.29° γ: 90°
R-values:
R R work R free
0.183 0.182 0.234
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

34 review citations

An updated evolutionary classification of CRISPR-Cas systems.
Makarova et al. (2015)
33 more

2 mentions without citation

Structure Principles of CRISPR-Cas Surveillance and Effector Complexes.
Tsui et al. (2015)
1 more