› 4qrx

X-ray diffraction
3.14Å resolution

Crystal structure of pro-papain mutant at pH 4.0

Released:
DOI: 10.2210/pdb4qrx/pdb
PDB entry 4qrx coloured by chain, front view.
PDB entry 4qrx coloured by chain, side view.
PDB entry 4qrx coloured by chain, top view.
Source organism: Carica papaya
Entry authors: Dutta S, Choudhury D, Roy S, Biswas S

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Papain Chains: A, C
Molecule details ›
Chains: A, C
Length: 363 amino acids
Theoretical weight: 41.07 KDa
Source organism: Carica papaya
Expression system: Escherichia coli
UniProt:
  • Canonical: P00784 (Residues: 27-345; Coverage: 98%)
Sequence domains:
Structure domains: Cysteine proteinases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 42.592Å b: 74.212Å c: 116.233Å
α: 90° β: 92.46° γ: 90°
R-values:
R R work R free
0.167 0.162 0.254
Expression system: Escherichia coli

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