› 4qt4

X-ray diffraction
2.19Å resolution

Crystal structure of Peptidyl-tRNA hydrolase from a Gram-positive bacterium, Streptococcus pyogenes at 2.19 Angstrom resolution shows the Closed Structure of the Substrate Binding Cleft

Released:
DOI: 10.2210/pdb4qt4/pdb
PDB entry 4qt4 coloured by chain, front view.
PDB entry 4qt4 coloured by chain, side view.
PDB entry 4qt4 coloured by chain, top view.
Source organism: Streptococcus pyogenes NZ131
Primary publication:
Crystal structure of peptidyl-tRNA hydrolase from a Gram-positive bacterium, Streptococcus pyogenes at 2.19 Å resolution shows the closed structure of the substrate-binding cleft.
Singh A, Gautam L, Sinha M, Bhushan A, Kaur P, Sharma S, Singh TP
FEBS Open Bio 4 915-22 (2014)
PMID: 25389518

Function and Biology Details

Reaction catalysed: 
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-tRNA hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 21.22 KDa
Source organism: Streptococcus pyogenes NZ131
Expression system: Escherichia coli
UniProt:
  • Canonical: B5XIP6 (Residues: 1-189; Coverage: 100%)
Gene names: Spy49_0005, pth
Sequence domains: Peptidyl-tRNA hydrolase
Structure domains: Peptidyl-tRNA hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P1
Unit cell:
a: 36.024Å b: 43.028Å c: 65.1Å
α: 90.33° β: 105.78° γ: 112.51°
R-values:
R R work R free
0.17 0.168 0.198
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Unraveling the stereochemical and dynamic aspects of the catalytic site of bacterial peptidyl-tRNA hydrolase.
Kabra et al. (2017)
5 more

2 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
1 more