› 4qtc

X-ray diffraction
1.4Å resolution

Structure of human haspin (GSG2) in complex with SCH772984 revealing the first type-I binding mode

Released:
DOI: 10.2210/pdb4qtc/pdb
PDB entry 4qtc coloured by chain, front view.
PDB entry 4qtc coloured by chain, side view.
PDB entry 4qtc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A unique inhibitor binding site in ERK1/2 is associated with slow binding kinetics.
Chaikuad A, Tacconi EM, Zimmer J, Liang Y, Gray NS, Tarsounas M, Knapp S
Nat Chem Biol 10 853-60 (2014)
PMID: 25195011

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase haspin Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8TF76 (Residues: 465-798; Coverage: 42%)
Gene names: GSG2, HASPIN
Sequence domains: Haspin like kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MPD 1 x MPD
Ligand GOL 2 x GOL
Ligand 38Z 1 x 38Z
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 77.89Å b: 78.36Å c: 81.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.164
Expression system: Escherichia coli BL21

Quick links

Citations

19 review citations

Targeting RAS-ERK signalling in cancer: promises and challenges.
Samatar et al. (2014)
18 more

2 mentions without citation

Emerging roles of the αC-β4 loop in protein kinase structure, function, evolution, and disease.
Yeung et al. (2020)
1 more