› 4qts

X-ray diffraction
3.1Å resolution

Crystal structure of Csm3-Csm4 subcomplex in the type III-A CRISPR-Cas interference complex

Released:
DOI: 10.2210/pdb4qts/pdb
PDB entry 4qts coloured by chain, front view.
PDB entry 4qts coloured by chain, side view.
PDB entry 4qts coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Crystal structure of the Csm3-Csm4 subcomplex in the type III-A CRISPR-Cas interference complex.
Numata T, Inanaga H, Sato C, Osawa T
J Mol Biol 427 259-73 (2015)
PMID: 25451598

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176775 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CRISPR system Cms protein Csm4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 376 amino acids
Theoretical weight: 43.92 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59062 (Residues: 1-376; Coverage: 100%)
Gene names: MJ1668, csm4
Sequence domains:
CRISPR system Cms endoribonuclease Csm3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 268 amino acids
Theoretical weight: 30.38 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59063 (Residues: 1-248; Coverage: 100%)
Gene names: MJ1669, csm3
Sequence domains: RAMP superfamily

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 74.653Å b: 96.319Å c: 194.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.288
Expression system: Escherichia coli

Quick links

Citations

2 review citations

DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes.
Plagens et al. (2015)
1 more

4 mentions without citation

Type III-A CRISPR-Cas Csm Complexes: Assembly, Periodic RNA Cleavage, DNase Activity Regulation, and Autoimmunity.
Jia et al. (2019)
3 more