› 4quv

X-ray diffraction
2.74Å resolution

Structure of an integral membrane delta(14)-sterol reductase

Released:
DOI: 10.2210/pdb4quv/pdb
PDB entry 4quv coloured by chain, front view.
PDB entry 4quv coloured by chain, side view.
PDB entry 4quv coloured by chain, top view.
Source organism: Methylotuvimicrobium alcaliphilum 20Z
Primary publication:
Structure of an integral membrane sterol reductase from Methylomicrobium alcaliphilum.
Li X, Roberti R, Blobel G
Nature 517 104-7 (2015)
PMID: 25307054

Function and Biology Details

Reaction catalysed: 
4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP(+) = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-124445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta(14)-sterol reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 49.47 KDa
Source organism: Methylotuvimicrobium alcaliphilum 20Z
Expression system: Escherichia coli
UniProt:
  • Canonical: G4SW86 (Residues: 1-427; Coverage: 100%)
Gene names: MEALZ_1312, erg
Sequence domains: Ergosterol biosynthesis ERG4/ERG24 family

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 74.66Å b: 74.615Å c: 79.549Å
α: 66° β: 90.37° γ: 86.86°
R-values:
R R work R free
0.236 0.233 0.284
Expression system: Escherichia coli

Quick links

Citations

4 review citations

DHCR7: A vital enzyme switch between cholesterol and vitamin D production.
Prabhu et al. (2016)
3 more

16 mentions without citation

Structural basis for catalysis at the membrane-water interface.
Dufrisne et al. (2017)
15 more