› 4r0a

X-ray diffraction
1.9Å resolution

Crystal structure of human TLR8 in complex with uridine mononucleoside

Released:
DOI: 10.2210/pdb4r0a/pdb
PDB entry 4r0a coloured by chain, front view.
PDB entry 4r0a coloured by chain, side view.
PDB entry 4r0a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Toll-like receptor 8 senses degradation products of single-stranded RNA.
Tanji H, Ohto U, Shibata T, Taoka M, Yamauchi Y, Isobe T, Miyake K, Shimizu T
Nat Struct Mol Biol 22 109-15 (2015)
PMID: 25599397

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Toll-like receptor 8 Chain: A
Molecule details ›
Chain: A
Length: 811 amino acids
Theoretical weight: 92.84 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q9NR97 (Residues: 27-827; Coverage: 79%)
Gene names: TLR8, UNQ249/PRO286
Sequence domains: Leucine rich repeat
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Ligand NAG 2 x NAG
Ligand BMA 1 x BMA
Ligand MAN 2 x MAN
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand URI 1 x URI
Ligand NAG 7 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2221
Unit cell:
a: 105.444Å b: 133.319Å c: 120.039Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.2 0.26
Expression system: Drosophila melanogaster

Quick links

Citations

83 review citations

Toll-like Receptors and the Control of Immunity.
Fitzgerald et al. (2020)
82 more

6 mentions without citation

Structural and functional understanding of the toll-like receptors.
Asami et al. (2021)
5 more