› 4r0m

X-ray diffraction
2.45Å resolution

Structure of McyG A-PCP complexed with phenylalanyl-adenylate

Released:
DOI: 10.2210/pdb4r0m/pdb
PDB entry 4r0m coloured by chain, front view.
PDB entry 4r0m coloured by chain, side view.
PDB entry 4r0m coloured by chain, top view.
Source organism: Microcystis aeruginosa PCC 7806
Primary publication:
Structure of the adenylation-peptidyl carrier protein didomain of the Microcystis aeruginosa microcystin synthetase McyG.
Tan XF, Dai YN, Zhou K, Jiang YL, Ren YM, Chen Y, Zhou CZ
Acta Crystallogr D Biol Crystallogr 71 873-81 (2015)
PMID: 25849398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108816 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
McyG protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 643 amino acids
Theoretical weight: 73.12 KDa
Source organism: Microcystis aeruginosa PCC 7806
Expression system: Escherichia coli
UniProt:
  • Canonical: A8YJW1 (Residues: 1-643; Coverage: 24%)
Gene names: IPF_370, mcyG
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FA5 2 x FA5
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 120.71Å b: 120.71Å c: 262.78Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.248 0.247 0.273
Expression system: Escherichia coli

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Citations

6 review citations

The structural biology of biosynthetic megaenzymes.
Weissman KJ. (2015)
5 more

2 mentions without citation

Structural advances toward understanding the catalytic activity and conformational dynamics of modular nonribosomal peptide synthetases.
Patel et al. (2023)
1 more