› 4r1z

X-ray diffraction
3.3Å resolution

Function and Biology Details

Reactions catalysed: 
A C(21)-steroid + [reduced NADPH--hemoprotein reductase] + O(2) = a 17-alpha-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H(2)O
17-alpha-hydroxypregnenolone + [reduced NADPH--hemoprotein reductase] + O(2) = 3-beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-109806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid 17-alpha-hydroxylase/17,20 lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 439 amino acids
Theoretical weight: 49.6 KDa
Source organism: Danio rerio
UniProt:
  • Canonical: B3DH80 (Residues: 81-519; Coverage: 88%)
Gene name: cyp17a1
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand AER 2 x AER
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3121
Unit cell:
a: 136.63Å b: 136.63Å c: 135.441Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.168 0.238

Quick links

Citations

5 review citations

Steroid 17-hydroxylase and 17,20-lyase deficiencies, genetic and pharmacologic.
Auchus RJ. (2017)
4 more

5 mentions without citation

Recent Structural Insights into Cytochrome P450 Function.
Guengerich et al. (2016)
4 more