› 4r21

X-ray diffraction
2.7Å resolution

Zebra fish cytochrome P450 17A2 with Progesterone

Released:
DOI: 10.2210/pdb4r21/pdb
PDB entry 4r21 coloured by chain, front view.
PDB entry 4r21 coloured by chain, side view.
PDB entry 4r21 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structural and kinetic basis of steroid 17α,20-lyase activity in teleost fish cytochrome P450 17A1 and its absence in cytochrome P450 17A2.
Pallan PS, Nagy LD, Lei L, Gonzalez E, Kramlinger VM, Azumaya CM, Wawrzak Z, Waterman MR, Guengerich FP, Egli M
J Biol Chem 290 3248-68 (2015)
PMID: 25533464

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108403 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450, family 17, subfamily A, polypeptide 2 precursor Chains: A, B
Molecule details ›
Chains: A, B
Length: 486 amino acids
Theoretical weight: 54.15 KDa
Source organism: Danio rerio
UniProt:
  • Canonical: A7U483 (Residues: 26-495; Coverage: 100%)
Gene name: cyp17a2
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand STR 2 x STR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 55.94Å b: 78.63Å c: 95.41Å
α: 90° β: 92.03° γ: 90°
R-values:
R R work R free
0.209 0.205 0.283

Quick links

Citations

5 review citations

Steroid 17-hydroxylase and 17,20-lyase deficiencies, genetic and pharmacologic.
Auchus RJ. (2017)
4 more

3 mentions without citation

Recent Structural Insights into Cytochrome P450 Function.
Guengerich et al. (2016)
2 more