4r28

X-ray diffraction
3.06Å resolution

MspJI Restriction Endonuclease in Complex with 27-mer Oligonucleotide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-102225 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Restriction endonuclease Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 456 amino acids
Theoretical weight: 49.79 KDa
Source organism: Mycobacterium sp. JLS
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0J9X157 (Residues: 1-456; Coverage: 100%)
Gene name: Mjls_0822
Sequence domains:
Structure domains:
DNA (25-MER) Chain: X
Molecule details ›
Chain: X
Length: 27 nucleotides
Theoretical weight: 8.37 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (26-MER) Chain: Y
Molecule details ›
Chain: Y
Length: 27 nucleotides
Theoretical weight: 8.24 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P61
Unit cell:
a: 88.544Å b: 88.544Å c: 511.971Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.227 0.258
Expression systems:
  • Escherichia coli
  • Not provided