› 4r2f

X-ray diffraction
2Å resolution

Crystal structure of sugar transporter ACHL_0255 from Arthrobacter chlorophenolicus A6, target EFI-510633, with bound laminaribiose

Released:
DOI: 10.2210/pdb4r2f/pdb
PDB entry 4r2f coloured by chain, front view.
PDB entry 4r2f coloured by chain, side view.
PDB entry 4r2f coloured by chain, top view.
Source organism: Pseudarthrobacter chlorophenolicus A6
Entry authors: Patskovsky Y, Toro R, Bhosle R, Al Obaidi N, Chamala S, Attonito JD, Scott Glenn A, Chowdhury S, Lafleur J, Siedel RD, Hillerich B, Love J, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110724 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Extracellular solute-binding protein family 1 Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 46.13 KDa
Source organism: Pseudarthrobacter chlorophenolicus A6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8H997 (Residues: 31-438; Coverage: 100%)
Gene name: Achl_0255
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6322
Unit cell:
a: 139.101Å b: 139.101Å c: 96.458Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.165 0.207
Expression system: Escherichia coli BL21(DE3)

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