› 4r4v

X-ray diffraction
3.07Å resolution

Crystal structure of the VS ribozyme - G638A mutant

Released:
DOI: 10.2210/pdb4r4v/pdb
PDB entry 4r4v coloured by chain, front view.
PDB entry 4r4v coloured by chain, side view.
PDB entry 4r4v coloured by chain, top view.
Source organism: Neurospora
Primary publication:
Crystal structure of the Varkud satellite ribozyme.
Suslov NB, DasGupta S, Huang H, Fuller JR, Lilley DM, Rice PA, Piccirilli JA
Nat Chem Biol 11 840-6 (2015)
PMID: 26414446

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196864 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
VS ribozyme RNA Chain: A
Molecule details ›
Chain: A
Length: 186 nucleotides
Theoretical weight: 60.17 KDa
Source organism: Neurospora
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 9 x MG
Ligand K 5 x K
1 modified residue:
Ligand GDP 1 x GDP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 102.229Å b: 102.229Å c: 215.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.204
Expression system: Not provided

Quick links

Citations

11 review citations

Structure-based mechanistic insights into catalysis by small self-cleaving ribozymes.
Ren et al. (2017)
10 more

15 mentions without citation

Structural Insights into RNA Dimerization: Motifs, Interfaces and Functions.
Bou-Nader et al. (2020)
14 more