› 4r6i

X-ray diffraction
2.65Å resolution

AtxA protein, a virulence regulator from Bacillus anthracis.

Released:
DOI: 10.2210/pdb4r6i/pdb
PDB entry 4r6i coloured by chain, front view.
PDB entry 4r6i coloured by chain, side view.
PDB entry 4r6i coloured by chain, top view.
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Primary publication:
Crystal structure of Bacillus anthracis virulence regulator AtxA and effects of phosphorylated histidines on multimerization and activity.
Hammerstrom TG, Horton LB, Swick MC, Joachimiak A, Osipiuk J, Koehler TM
Mol Microbiol 95 426-41 (2015)
PMID: 25402841

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175033 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthrax toxin expression trans-acting positive regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 478 amino acids
Theoretical weight: 56.33 KDa
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q44636 (Residues: 1-475; Coverage: 100%)
Gene names: BXA0146, GBAA_pXO1_0146, atxA, pXO1-119
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand LMT 2 x LMT
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 130.46Å b: 135.167Å c: 180.908Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.252
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Regulatory gene mutation: a driving force behind group a Streptococcus strain- and serotype-specific variation.
Sarkar et al. (2017)
2 more

5 mentions without citation

PRD-Containing Virulence Regulators (PCVRs) in Pathogenic Bacteria.
Rom et al. (2021)
4 more